Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge

Ivis detector for the analytical ultracentrifuge

A multiwavelength UV/vis detector for the analytical ultracentrifuge (MWL-AUC) has been developed recently. In this work, [3-carotene-gelatin composite particles are investigated with MWL-AUC. Band centrifugation with a Vinograd cell is used to ensure maximum sample separation. Spectral changes of the system are observed in dependence of the sedimentation coefficient and are attributed to a pre...

The analytical ultracentrifuge spins again

Uppsala University, BiomedicalCenter, p. O. Box 574, 5-751 23 Uppsala, Sweden. Goran Pettersson /b at the Department of Biochemistry, Uppsala University, Biomedical Center, P. O. Box 576, 5-751 23 Uppsala, Sweden. Ingrid Marle is at Pharmaceutical and Analytical R&D, Astra Pain ControlAB, S-151 85 Sodertdlje, Sweden. Jerry Stdlberg is at the Department of Molecular Biology, Uppsala University, ...

Self-associating Systems in the Analytical Ultracentrifuge

A simple test for macromolecular heterogeneity in the analytical ultracentrifuge.

A simple check for the presence of heterogeneity in a macromolecular system is proposed, employing comparison of Rayleigh sedimentation-equilibrium patterns for two solutions of the same fringe concentration but differing absolute concentrations. The method is illustrated by application to a bronchial glycoprotein from a cystic-fibrosis patient.

Challenges for the modern analytical ultracentrifuge analysis of polysaccharides.

This article reviews some of the recent advances in analytical ultracentrifugation and how these advances have impacted--and can impact--on our understanding of the size, shape through conformation modelling, interactions and charge properties of polysaccharides in solution, particularly when used in combination with other solution techniques and also imaging techniques. Specifically we look at...